| Application |
The Universal Mycoplasma Detection Kit offers a quick and sensitive PCR-based test to detect mycoplasma contaminants in cell culture. All components required for the PCR reaction are provided and have been optimized for amplification. High specificity is obtained through the utilization of a proprietary mix of buffers, dNTPs and thermostable polymerase, combined with universal primers that are specific to the 16S rRNA coding region in the mycoplasma genome. DNA originating from other sources, such as tissue samples or other bacteria, are not amplified. A touchdown PCR regimen increases sensitivity of the assay, along with enhancing specificity.
The kit detects over 60 species of Mycoplasma, Acholeplasma, Spiroplasma and Ureaplasma including the eight species most likely to afflict cell cultures: M. arginini, M. fermentans, M. hominis, M.hyorhinis, M. orale, M. pirum, M. salivarium, and A. laidlawii. Samples that are positive for mycoplasma are easily recognized by a distinct PCR product ranging in size from 434 to 468 bp on an agarose gel.
| Component |
| Type Strain |
Volume |
| Biosafety Level |
Composition |
| Product Format |
Storage |
| Storage Conditions |
Lysis Buffer |
| 梅經(jīng)理 |
17280875617 |
1438578920 |
| 胡經(jīng)理 |
13345964880 |
2438244627 |
| 周經(jīng)理 |
17757487661 |
1296385441 |
| 于經(jīng)理 |
18067160830 |
2088210172 |
| 沈經(jīng)理 |
19548299266 |
2662369050 |
| 李經(jīng)理 |
13626845108 |
972239479 |
沧源|
深州市|
丰台区|
乐安县|
义马市|
仁化县|
岳池县|
龙川县|
巴林左旗|
渝中区|
诏安县|
洪湖市|
宜城市|
治多县|
休宁县|
岳阳县|
藁城市|
杭锦后旗|
沙湾县|
中阳县|
马公市|
郑州市|
鄂州市|
博野县|
京山县|
丰县|
闽清县|
花垣县|
遵义县|
紫金县|
韶山市|
东海县|
彰化县|
蓬安县|
石门县|
南昌县|
调兵山市|
故城县|
蒲城县|
施秉县|
蒲江县|
|
