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produces protein interleukin 2

Construct size (kb): 6.199999809265137

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pPL-lambda
Intact vector size: 5.218
Type of vector: plasmid
Vector end: HpaI
Vector end: HpaI
Cloning sites: HpaI
Polylinker sites:
Construction: pBR322, lambda
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: lambdaPL
replicon: pMB1
terminator: lambda tL1
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: bovine
Gene symbol: IL2
Genomic copy number: unique
Gene name: interleukin 2
Contains complete coding sequence?: U
Tissue: lymph node, Con A-stimulated
Type of DNA: cDNA
Insert end: HgiAI
Insert end: PstI
Insert size (kb): 0.75
Cross references: DNA Seq. Acc.: M12791

Insert lengths(kb): 0.75

Tissue: lymph node, Con A-stimulated

Gene product: interleukin 2 [IL2]

Target Gene: interleukin 2

Restriction digests of pLNbovIL-2 give the following sizes (kb): EcoRI--6.2; SalI--6.2; NcoI--6.2; XbaI--6.0, 0.2; XbaI/SphI--4.3, 1.7, 0.2.

Restriction digests of pRK248cIts give the following sizes (kb): EcoRI--11.0; SalI--11.0; NcoI--10.5, 0.5; XbaI--uncut; SphI--11.0.

Contains 2 plasmids: pLNbovIL2 (6.2 kb, ampicillin resistant) and pRK248cIts (ATCC 33766) (11.0 kb, tetracycline resistant), encoding the temperature-sensitive lambda cI repressor used to regulate expression from pLNbovIL2.

pRK248cIts is present at lower copy number and can usually be distinguished from pLNbovIL-2 on agarose gels stained with ethidium bromide.

Detects bovine genomic restriction fragments of the following sizes (kb): BamHI--10.0; EcoRI--5.8, 5.0; HindIII--5.1, 3.3, 1.9.

After thermal induction, this construct produces biologically active product.

The HgiAI/PstI fragment encoding aa 22 to 135 from pIL-2-4 was blunt-ended and inserted into a construct based on pPL-lambda already containing an oligonucleotide providing a ribosome binding site, an initiator Met codon, and a codon for Ala 21.