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West Nile virus
West Nile virus
規(guī)格:
貨期:
編號:B162858
品牌:Mingzhoubio

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產(chǎn)品名稱 West Nile virus
商品貨號 B162858
Classification Flaviviridae, Flavivirus
Agent West Nile virus
Strain Monoclonal antibody to West Nile virus Envelope Protein, Clone E121 (produced in vitro)
Common Name West Nile virus
Applications
Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E121 hybridoma supernatant by protein G affinity chromatography.
The antibody is reported to be reactive using flow cytometry and Western blot analysis.
Emerging infectious disease research
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E121 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E121 antibody is reported to bind to the lateral ridge of domain I in the envelope glycoprotein.
Product Format frozen Each vial of VR-1618 contains approximately 100 µL of purified monoclonal antibody in PBS.
Storage Conditions ?Store at -20°C or colder immediately upon arrival Repeated freeze-thaw cycles should be avoided
Comments
Antibody Class: IgG2aκ
Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E121 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E121 antibody is reported to bind to the lateral ridge of domain I in the envelope glycoprotein.
Functional activity is confirmed by reactivity in immunofluorescence assays using Vero cells (ATCC CCL-81) infected with WNV and by ELISA using WNV-infected cell lysates [WNV, Eg101 (Egypt 1951)]. The antibody is reported to be reactive using flow cytometry and Western blot analysis.
Effect on Host
Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E121 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E121 antibody is reported to bind to the lateral ridge of domain I in the envelope glycoprotein.
Name of Depositor MS Diamond
References

Oliphant T, et al. Antibody Recognition and Neutralization Determinants on Domains I and II of West Nile Virus Envelope Protein. J. Virol. 80:12149-12159, 2006. PubMed:17035317.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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