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Construct size (kb): 4.96999979019165

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcD-X
Intact vector size: 2.800
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Features (with orientation and position when available):

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MMP1
Other gene symbols: CLG
Gene name: matrix metalloproteinase 1 (interstitial collagenase)
Contains complete coding sequence?: Y
Chromosome: 11; Localization: 11 q21-q22
Strain: A2058 melanoma cell line
Tissue: brain metastasis
Type of DNA: cDNA
Insert 5' end: Modification: GC
Insert 3' end: Modification: AT
Insert size (kb): 2.2
Cross references: DNA Seq. Acc.: M13509

Insert lengths(kb): 2.200000047683716

Tissue: brain metastasis

Gene product: matrix metalloproteinase 1 (interstitial collagenase)(collagenase, epidermolysis bullosa, dystrophic, (autosomal recessive), collagenase, fibroblast, collagenase, interstitial) [CLG]

Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 1.45, 0.75; EcoRI--5.3; XhoI--3.0, 2.3; SacI--5.3; PstI--2.5, 1.5, 1.3.

The insert contains the following restriction sites (bp from the 5' end of the cDNA sequence): BamHI--1290; EcoRI--274; HincII--1951; SacI--1010; PstI--1468; XbaI--138, 1570.

The insert sequence is essentially identical to that reported for fibroblast collagenase except for noted changes.

Contains the complete coding sequence (nt 68-1478 of the cDNA). The 2.2 kb XhoI fragment includes some sequence from the vector: 175 bp at the 5' end and 50 bp at the 3' end.