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pV11SPRS [TRGV11]
pV11SPRS [TRGV11]
規(guī)格:
貨期:
編號:B240268
品牌:Mingzhoubio

標準菌株
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DNA
RNA

規(guī)格:
凍干粉
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甘油
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產(chǎn)品名稱 pV11SPRS [TRGV11]
商品貨號 B240268
Designations pV11SPRS [TRGV11]
GenBank Number

X07207

Species Homo sapiens, human
Depositors M Lefranc
Vector
Construct size (kb): 3.0
Insert
DNA: genomic
Insert lengths(kb): 0.2700000107288361
Gene product: T cell receptor, gamma, variable region V11 [TCRGV11]
Alleles: F2, F1, E1, E2
Insert Size (kb) 0.270
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 0.27; EcoRI--3.0; HindIII--3.0; RsaI--2.0, 0.66, 3.2; RsaI/HindIII--1.7, 0.66, 0.32(doublet).
Codominant segregation of the SacI polymorphism demonstrated in one family; demonstrated in 3 families for the TaqI polymorphism.
The A2 allele of the SacI polymorphism results from the presence of an additional SacI site at 5.8 kb in 3' of V11.
Run on 0.8% agarose gels and hybridize under low stringency conditions.
Subcloned from lambdaJM15. Contains the 5' region of the gene.
The probe detects the following invariant genomic fragments (kb): HindIII--19; EcoRI--11.2; PvuII--4.0.
References

Huck S, et al. Variable region genes in the human T-cell rearranging gamma (TRG) locus: V-J junction and homology with the mouse genes. EMBO J. 7: 719-726, 1988. PubMed: 2969332

Ghanem N, et al. SacI poplymorphism defined by human T-cell receptor gamma (TCRG) variable gene VII. Nucleic Acids Res. 17: 1271, 1989. PubMed: 2564181

Ghanem N, et al. A TaqI RFLP of the human T-cell receptor gamma (TCRG) variable gene V11. Nucleic Acids Res. 17: 1270, 1989. PubMed: 2564180

Lefranc MP, et al. Molecular mapping of the human T cell receptor gamma (TRG) genes and linkage of the variable and constant regions. Eur. J. Immunol. 19: 989-994, 1989. PubMed: 2526744

Alexandre D, et al. Reciprocal hybrid joints demonstrate successive V - J rearrangements on the same chromosome in the human TCR gamma locus. Int. Immunol. 3: 973-982, 1991. PubMed: 1836738

Forster A, et al. New subgroups in the human T cell rearranging Vgamma gene locus. EMBO J. 6: 1945-1950, 1987. PubMed: 2820713

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