產(chǎn)品名稱 |
Saccharomyces cerevisiae Meyen ex E.C. Hansen |
商品貨號(hào) |
B242576 |
Deposited As |
Saccharomyces cerevisiae Hansen, teleomorph |
Classification |
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
Strain Designations |
BY4718 |
Alternate State |
Candida robusta Diddens et Lodder |
Application |
transformation host with non-revertable mutations for auxotrophic markers transformation host with non-revertable designer deletions |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen |
Type Strain |
no |
Genotype |
MATa met15delta0 ura3delta0
RefBrachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 |
Preceptrol® |
no |
Mating Type |
a |
Ploidy |
haploid |
Comments |
One of a series of strains (ATCC 200866-200902) useful for gene disruption experiments. The strains differ in mating type and in non-revertable mutations for one or more of these auxotrophic markers: ade2delta::hisG, his3delta200, leu2delta0, lys2delta0, met15delta0, trp1delta63, ura3delta0. |
Morphology |
After 3 days on YEPD medium at 25°C, colonies are off-white, smooth, butyrous. Cells are ovoid or irregular; single, in pairs or small clusters. |
Medium |
ATCC® Medium 28: Emmons' modification of Sabouraud's agar
ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 1245: YEPD
|
Growth Conditions |
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic |
Sequenced Data |
No DNA sequencing was performed in house on this product.
|
Name of Depositor |
JD Boeke |
Special Collection |
NCRR Contract |
References |
Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801
Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801
|