The smaller EcoRI insert fragment has been used to detect the polymorphism.
Vector
Construct size (kb): 43.29999923706055
Insert
DNA: genomic
Insert lengths(kb): 3.849999904632568
Tissue: DNA from hamster/human 314-1b hybrid cell line
Gene product: DNA Segment, single copy [D3S617]
Alleles: A3, A1, A2, B1, B2, B3
Insert Size (kb)
3.850
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--20.0 (doublet), 5.6, 2.5; BglII--23.0, 6.8, 6.6, 3.5, 1.9, 0.95; EcoRI--21.0 (doublet), 2.5, 1.35; XhoI--23.0, 13.0.
D3S617 has been localized to 3pter-p25 on the human chromosome.
There is no evidence as to whether the 2 insert EcoRI fragments are contiguous in the genome.
The smaller EcoRI insert fragment has been used to detect the polymorphism.