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YIp353
YIp353
規(guī)格:
貨期:
編號:B244126
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 YIp353
商品貨號 B244126
Designations YIp353
Depositors CJ Lusty
Biosafety Level 1
Vector Information
Size (kb): 7.0999999046325680
Vector: YIp353 (plasmid)
Promoters: Promoter none
Construction: YEp353 (ATCC 37725), YIp352
Marker(s):URA3,ampR
Construct size (kb): 7.099999904632568
Features: insert detection: lacZ
marker(s): ampR, URA3
promoter: none
replicon: pMB1
Applications
YI-type (integrating) shuttle vector
promoter-cloning vector
shuttle vector
Comments
The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-2; SmaI-3; BamHI-3; SalI-3; PstI-1; HindIII-2.
Restriction digests of the clone give the following sizes (kb): EcoRI--7.1; BamHI--7.1; SalI--7.1; PstI--7.1; HindIII--7.1.
One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37743 - 37745) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.
The sequence and reading frame of the multiple cloning sequence is: 5' GA ATT CCC GGG GAT CCG TCG ACC TGC AGC CAA GCT TGC GAT CCC 3'. The sequence begins with the first nucleotide of the MCS and ends with the CCC for amino acid 8 of beta-galactosidase.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915

Shipped freeze-dried
Shipping Information

Shipped: ?Freeze dried ?E. coli containing the plasmid

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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