精品999日本久久久影院,中文字幕无码免费久久,东北少妇不带套对白,亚洲国产AV无码专区亚洲AVL,精品国产18久久久久久,无码无套少妇毛多69XXX,无码人妻免费一区二区三区,亚洲中文字幕无码中文字幕

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > Crypthecodinium cohnii (Seligo) Javornicky
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
Crypthecodinium cohnii (Seligo) Javornicky
Crypthecodinium cohnii (Seligo) Javornicky
規(guī)格:
貨期:
編號:B244573
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Crypthecodinium cohnii (Seligo) Javornicky
商品貨號 B244573
Strain Designations MB 3
Application
Biofuel production
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation Sargassum sp., Miami Beach, FL, 1977
Product Format frozen
Storage Conditions Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section
Type Strain no
Comments
Major Sibling Species Y
Medium ATCC® Medium 460: A2E6 medium
Growth Conditions
Temperature: 20°C to 25°C
Culture System: Axenic
Cryopreservation

Harvest and Preservation

  1. Harvest cells from cultures which are at or near peak density.  Aseptically transfer cells to 15 mL plastic centrifuge tubes and centrifuge at ~150 x g for 5 min.
  2. Adjust the concentration of cells to 2 x 106/mL with fresh medium, then dilute to half this concentration by adding an equal amount of a 15% (v/v) sterile glycerol solution in fresh  medium.
  3. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).  The time from mixing of the cell suspension and the glycerol solution, before the cooling cycle begins, should be no less than 15 min and no greater than 30 min.
  4. Place vials in a controlled-rate freezing unit. From room temperature, cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)   
  5. The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials can be stored between -80 and -70°C for no longer than one week.
  6. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.
  7. Immediately after thawing, do not leave in the water bath; aseptically remove the contents of the ampule and transfer to a fresh test tube (or T-25 flask) containing 5 mL of ATCC Medium 460. Incubate culture at 20-25°C (test tubes are incubated upright with the cap loosened one-half turn).  Subculture every 10-14d.
Name of Depositor CA Beam, M Himes
Year of Origin 1977
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
汉源县| 格尔木市| 连州市| 井冈山市| 武鸣县| 新干县| 马龙县| 于都县| 台北市| 金昌市| 阜城县| 葵青区| 靖边县| 团风县| 孟州市| 丹江口市| 长海县| 永丰县| 阳春市| 当雄县| 青海省| 凤台县| 洛川县| 娱乐| 读书| 古田县| 泰和县| 灵寿县| 峨山| 突泉县| 山东省| 广安市| 广灵县| 印江| 大厂| 罗山县| 定州市| 海宁市| 易门县| 中超| 宝坻区|